Belal Mahmood1, Avijit Das2, Fahmi Iqbal Rabbi3, Kaisar Talukder4, Ashraf-ul-Islam5
1. Professor and Head, Dept. of Microbiology, NEMC, Sylhet
2. Associate Professor. Dept. Microbiology, NEMC, Sylhet
3. Assistant Professor, Dept. of Microbiology, NEMC, Sylhet
4. Senior Scientist, Dept. of Enteric Lab, ICDDR,B, Dhaka
5. Medical Officer, Dept. of Paediatrics, NEMC, Sylhet
Abstract
The prevalence of extended-spectrum ẞ-lactamases (ESBL)- producing microorganism E.coli in the town of Sylhet was assessed over 12 month period.
One hundred patients who had symptomatic urinary tract infections were selected for this study and urine samples were collected from the private laboratories of the community. These isolates were further confirmed as ESBL-producing Escherichia coli by phenotypic methods.
The patient’s age ranged from 4 years to 60 years. The highest age incidence of UTI patient was 21-30 years (28%) followed by 31-40 years (20%). Patients having age range 41-50 years showed 15% and patients above 50 years constituted 19%.
The antibiotic susceptibility test for non- ESBL showed, 100% sensitivity to imipenem and meropenem. There were multidrug resistance to Amoxicillin-clavulanic acid (AMC) 30% Ceftazidime (CAZ) 40%, Ceftriaxone (CRO) 35%, and Ciprofloxacin (CIP) 60%. The sensitivity with AMC-70% CAZ-60%, CRO-65% & CIP-40%. The ESBL group were also 100% sensitivity to carbepenems and the rest of resistance percentage were AMC-80%, CAZ-55%, CRO-80%, CIP-80%.
The ESBL and non ESBL producing strains were separated for plasmid profile analysis. 60% of isolates exhibit high molecular wt plasmids (>140MDa) in ESBL groups and also in Non ESBL groups (64% ).These findings suggests that both ESBL and non ESBL producing isolates harbour large size plasmid, despite the fact that ESBL-producing strains cause wide range of multi-drug resistance in the community.
Key Words: Uropathogenic E.coli, ESBL E.coli, UTI, Echerichia coli.