Ismoth Ara Jerin1, Wajeunnesa2, Nahida sultana3, Jaber Ahmed Chowdhury4
Farhana Rahman5, Arfa Islam6
1. Assistant Professor, Department of Physiology, Jalalabad Ragib-Rabeya Medical college, Sylhet.
2. Associate Professor, Department of Physiology, Jalalabad Ragib-Rabeya Medical college, Sylhet
3. Assistant Professor, Department of Physiology, North East Medical College, Sylhet.
5. Assistant Professor, Department of Physiology, Sir Salimullah Medical College, Dhaka.
6. Assistant Professor, Department of Physiology, Sylhet Women’s Medical College, Sylhet.
Abstract
Nigella sativa (Kalozira) is a widely used medicinal plant throughout the world. Nigella sativa oil (NSO) suppressed pain in different animal models. The objective of this study was to assess the effects of Nigella sativa (Kalozira) oil against nociceptive pain after single administration with 3 increasing doses in rats. The antinociceptive effect was evaluated by tail immersion test. This experimental study was conducted in the Department of Physiology, Bangabandhu Sheikh Mujib Medical University (BSMMU), Dhaka, from March 2016 to February 2017. For this, 20 (twenty) Long Evans rats of both sexes were divided into control (A, 10 ml/kg of 1% solution of Tween 20) and experimental groups (B1. with 0.5 ml/kg NSO, B2, with 1 ml/kg NSO, B3, with 1.5 ml/kg NSO) with 5 rats in each group. All the agents were given intraperitoneally. To assess nociceptive pain, tail withdrawal latency was measured and antinociceptive effect was represented by percentage of maximum possible effect (MPE). Data were expressed as mean ± SEM (Standar Errer of Mean) and were statistically analyzed with SPSS using ANOVA, followed by Bonferroni post hoc test. In the interpretation of results p value 0.05 was considered as significant. The nociceptive pain was lowered by all three doses (0.5, 1, 1.5 ml/kg, i.p) in comparison to that of control group. NSO at doses of Iml/kg (p 0.001) and 1.5 ml/kg(p 0.001) was shown to be significantly more effective than that of 0.5 ml/kg (p 0.05). Therefore, it may be concluded that increasing the dose of Nigella sativa oil was progressively more effective in lowering nociceptive pain, as observed in this study.
Key Words: Pain, Nigella sativa, Tail immersion test.